Human OSM R beta Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human OSM R beta Affinity Purified Polyclonal Ab (25 UG)

货号： AF4389-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF4389-SP
别名	OSMR; OSMRB
全称	Oncostatin M Receptor beta
反应种属	Human
应用	Western Blot(0.1 µg/mL) Flow Cytometry(0.25 µg/10⁶cells)
目标/特异性	Detects human OSM R beta in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant mouse OSM R beta is observed.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 0.25 µg/10⁶cells
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	9180 (Human); 18414 (Mouse); 310132 (Rat)
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human OSM R beta Glu28-Ser739 Accession # Q99650
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	OSM R beta is a 150‑180 kDa member of the IL-6 receptor family. It associates with gp130 to form the type II OSM receptor that is responsive to OSM. The gp130 subunit is shared by other IL-6 family cytokine receptors (1, 2, 3, 4), and OSM R beta associates with gp130-like receptor (GPL) to form a receptor complex responsive to IL-31 (5, 6). The human OSM R beta cDNA encodes a 979 amino acid (aa) precursor that includes a 27 aa signal sequence, a 712 aa extracellular domain (ECD), a 22 aa transmembrane segment, and a 218 aa cytoplasmic domain. The ECD contains one partial and one complete hematopoietin domain, an Ig-like domain, and three fibronectin type-III domains. The cytoplasmic domain contains box1, 2, and 3 motifs (7). Within the ECD, human OSM R beta shares 55%, 58%, 61%, and 72% aa sequence identity with mouse, rat, bovine, and canine OSM R beta, respectively. It also shares 31% aa sequence identity with human LIF R, but less than 20% aa sequence identity with human CNTF R alpha, G-CSF R, IL-6 R, IL-11 R alpha, and TCCR. OSM R beta does not bind cytokines directly, but increases the affinity of gp130 for OSM, and GPL for IL-31 (7, 8). OSM R beta, gp130, and GPL each initiate signaling events following ligand stimulation (9, 10). Jak/STAT and MAPK pathways are activated by OSM R beta -containing receptors (9, 11, 12, 13), including STAT5b and SHC which are not activated by other IL-6 family receptors (10, 13). In mice, the loss of OSM R beta expression blocks erythroid progenitor development in bone marrow, and dramatically reduces the number of circulating platelets and erythrocytes (14). The type II OSM receptor is the only IL-6 family receptor that promotes osteoblast differentiation in calvaria cell cultures (15).
运输条件	Blue Ice
存放说明	4℃
参考文献	Chen, S.-H. and E.N. Benveniste (2004) Cytokine Growth Factor Rev. 15:379. Heinrich, P.C. et al. (2003) Biochem. J. 374:1. Tanaka, M. and A. Miyajima (2003) Rev. Physiol. Biochem. Pharmacol. 149:39. Gearing, D.P. et al. (1992) Science 255:1434. Dillon, S.R. et al. (2004) Nat. Immunol. 5:752. Diveu, C. et al. (2003) J. Biol. Chem. 278:49850. Mosley, B. et al. (1996) J. Biol. Chem. 271:32635. Diveu, C. et al. (2004) Eur. Cytokine Netw. 15:291. Dreuw, A. et al. (2004) J. Biol. Chem. 279:36112. Wang, Y. et al. (2000) J. Biol. Chem. 275:25273. Hermanns, H.M. et al. (2000) J. Biol. Chem. 275:40742. Kuropatwinski, K.K. et al. (1997) J. Biol. Chem. 272:15135. Auguste, P. et al. (1997) J. Biol. Chem. 272:15760. Tanaka, M. et al. (2003) Blood 102:3154. Malaval, L. et al. (2005) J. Cell. Physiol. 204:585.

参考图片

Detection of OSM R beta in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Goat Anti-Human OSM R beta Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4389, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).