| 货号 | AF4366-SP |
| 别名 | IB1; MAPK8IP1; PRKM8IP | 全称 | JNK-interacting Protein 1 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects endogenous human, mouse, and rat JIP1 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9479 (Human); 19099 (Mouse); 116457 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. LC3 binding to the scaffolding protein JIP1 regulates processive dynein-driven transport of autophagosomes. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human JIP1 Met531-Asp666 Accession # Q6NUQ9 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | JNK-interacting proteins (JIPs) comprise a family of four scaffolding proteins that tether and assemble components of the JNK signaling cascade. JIP1, also known as islet-brain 1 (IB1) and mitogen-activated protein kinase 8-interacting protein 1 (MAPK8IP1), contains an N-terminal JNK-binding domain, and C-terminal MLK- and MKK7-binding domains. JIP1 is ubiquitously expressed, with highest levels in brain and in pancreatic beta -cells. Mice lacking JIP1 are resistant to diet-induced obesity and show reduced diet-induced insulin resistance. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse and Rat JIP1 by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line, Neuro‑2A mouse neuroblastoma cell line, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat JIP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4366) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for JIP1 at approximately 105 to 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
