| 货号 | AF4345-SP |
| 别名 | EC 3.1.1.2; EC 3.1.1.81; EC 3.1.8.1; paraoxanase-3; paraoxonase 3; serum paraoxonase/lactonase 3 | 全称 | Paraoxonase 3 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects endogenous human, mouse, and rat PON3 in Western blots. In Western blots, this antibody did not cross‑react with recombinant human PON1 or PON2. |
| 使用方法 | Western Blot: 0.5 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5446 (Human); 269823 (Mouse); 312086 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. PON3 is upregulated in cancer tissues and protects against mitochondrial superoxide-mediated cell death. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human PON3 Ala30-Leu354 Accession # Q15166 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The paraoxonase (PON) gene family of antioxidant enzymes includes three known members located adjacent to each other on chromosome 7. Paraoxonase/lactonase 3 (PON3) is a 354 amino acid, 39.6 kDa protein that is secreted into the bloodstream and associates with high-density lipoprotein (HDL). PON3 has lactonase activity (EC 3.1.1) and rapidly hydrolyzes lactones. PON3 exhibits no paraoxonase and very limited arylesterase activities. PON3 is an important antioxidant and can inhibit the oxidation of low-density lipoprotein (LDL), a function that may slow the initiation and progression of atherosclerosis. PON3 is glycosylated at Asn323. Alternatively spliced variants encoding different protein isoforms have been described; however, only one has been fully characterized. Human PON3 shares 81% amino acid identity with mouse and rat PON3. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse/Rat PON3 by Western Blot. Western blot shows human serum, lysates of A549 human lung carcinoma cell line, and HepG2 human hepatocellular carcinoma cell line, mouse liver and spleen tissue, and rat liver tissue. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat PON3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4345) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PON3 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. |
