| 货号 | AF4340-SP |
| 别名 | EC 3.1.1; EC 3.1.1.1; KIAA1070neuroligin-1; MGC45115; neuroligin 1; NL1; Nlg; NLGN1 |
| 反应种属 | Human/Rat |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects recombinant rat Neuroligin 1/NLGN1 in direct ELISAs. Detects human and rat Neuroligin 1/NLGN1 in Western blots. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 22871 (Human); 192167 (Mouse); 116647 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant rat Neuroligin 1/NLGN1 Gln46-Leu696 Accession # Q62765 |
| 生物活性 | Human, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Neuroligin 1 (neurexin ligand 1; also NL1) is a 116 kDa member of the type-B carboxyesterase/lipase family of proteins. It is postsynaptically expressed on neurons and initiates excitatory presynapse maturation through binding to a select isoform of beta -neurexin. Mature rat NL1 is 798 amino acids (aa) in length. It is expressed as a type I transmembrane glycoprotein that contains a 652 aa extracellular domain (ECD) (aa 46‑697) and a 125 aa cytoplasmic tail. The ECD possesses a nonfunctional esterase homology domain (aa 54‑612). Rat NL1 has at least three alternate splice forms. One shows a deletion of aa 165‑184 plus aa 298‑306, while the remaining two show deletions of either aa 165‑184, or aa 298‑306, respectively. Rat ECD shares 97% and 93% aa identity with mouse and human NL1 CED, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human and Rat Neuroligin 1/ NLGN1 by Western Blot. Western blot shows lysates of rat embryonic cortical neurons, rat embryonic hippocampal neurons, and human brain (cortex) tissue. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti‑Rat Neuroligin 1/ NLGN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4340) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Neuroligin 1/NLGN1 at approximately 117 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
Neuroligin 1/NLGN1 in Rat Brain. Neuroligin 1/NLGN1 was detected in perfusion fixed frozen sections of rat brain (cortex) using Sheep Anti‑Rat Neuroligin 1/ NLGN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4340) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to neuronal cell bodies, processes, and dendritic spines. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. |
