| 货号 | AF4339-SP |
| 别名 | ADRBK1; adrenergic, beta, receptor kinase 1; BARK; BARK1; BARK1beta-ARK-1; Beta-ARK1; Beta-ARK-1; EC 2.7.11; EC 2.7.11.15; FLJ16718; G-protein coupled receptor kinase 2; GRK2beta-adrenergic receptor kinase 1 | 全称 | G protein-coupled Receptor Kinase 2 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects endogenous human, mouse, and rat GRK2. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 156 (Human); 110355 (Mouse); 25238 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human GRK2 Pro468-Leu689 Accession # P25098 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | G protein-coupled receptor kinases (GRKs) are important modulators of G protein-coupled receptor (GPCR) signaling. Receptor phosphorylation by specific GRKs plays a key role in triggering rapid desensitization. The GRK family consists of 7 isoforms that share a central catalytic domain with homology to other serine/threonine kinases. The catalytic domain is flanked by an amino-terminal RGS domain of 183‑188 amino acids and a carboxyl-terminus of variable length. GRK2 is a member of the beta ARK subfamily, and was originally termed beta -adrenergic receptor kinase 1 ( beta ARK1). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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GRK2 in Human Alzheimers Disease Brain. GRK2 was detected in immersion fixed paraffin-embedded sections of human Alzheimers disease brain using Human/Mouse/Rat GRK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4339) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
Detection of Human/Mouse/Rat GRK2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, BaF3 mouse pro‑B cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat GRK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4339) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GRK2 at approximately 79 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
