| 货号 | AF4338-SP |
| 别名 | h-Numb; mnb; m-numb; numb (Drosophila) homolog; numb homolog (Drosophila); protein numb homolog; Protein S171; S171 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Numb in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8650 (Human); 18222 (Mouse); 29419 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Numb Thr356-Leu592 Accession # AAH68476 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Numb is a 75 kDa member of a family of cytoplasmic adaptor proteins termed CLASPS (clathrin-associated sorting proteins). These link transmembrane cargo proteins to the endocytic machinery. Numb is normally associated with the basolateral surface of polarized cells and has been shown to regulate cell fate by modulating activity of the Notch pathway. Human Numb is 651 amino acids (aa) in length. It contains one phosphotyrosine-binding domain (PTB; aa 24‑172) and one proline-rich region (PRR; aa 366‑413). There is a potential for multiple alternate splice forms that involve all regions of the molecule. Over aa 356‑592, human Numb shares 88% and 90% aa identity with mouse and canine Numb, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Numb by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Numb Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4338) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Numb at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
