| 货号 | AF4337-SP |
| 别名 | BCTL1; Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor classA domains protein 1; BTCL1; BTCL1BCTL1neuropilin and tolloid-like protein 1; FLJ41325; neuropilin (NRP) and tolloid (TLL)-like 1 | 全称 | Neuropilin [NRP] And Tolloid [TLL]-like 1 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human NETO1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human NETO2 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 81832 (Human); 246317 (Mouse); 307206 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human NETO1 Thr23-Val345 Accession # Q8TDF5 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Neuropilin and tolloid-like protein 1 (NETO1), also known as Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1 (BTCL1), is a type I transmembrane protein that is expressed in the brain and retina. The 511 amino acid residue mature human NETO1 contains an extracellular domain with two CUB domains (aa 41‑155 and 172‑287), and one class A LDL-receptor segment (aa 292‑326). Three human NETO1 splice isoforms exist, including a secreted soluble variant that shows an Asp-to-Glu transition at residue 157 followed by a premature truncation. Over aa 23‑345, human NETO1 shares 97% and 98% aa identity with mouse and canine NETO1, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
NETO1 in MOLT‑4 Human Cell Line. NETO1 was detected in immersion fixed MOLT‑4 human acute lymphoblastic leukemia cell line using Sheep Anti-Human NETO1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4337) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. | |
NETO1 in Human Prostate. NETO1 was detected in immersion fixed paraffin-embedded sections of human prostate using Sheep Anti-Human NETO1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4337) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
