| 货号 | AF4335-SP |
| 别名 | EC 2.4.2.12; MGC117256; NAmPRTase; NAMPT; nicotinamide phosphoribosyltransferase; NMPRTase; PBEF1; PBEF1110035O14Rik; PBEF1DKFZp666B131; Pre-B cell-enhancing factor; pre-B-cell colony enhancing factor 1; Pre-B-cell colony-enhancing factor 1; VF; Visfatin | 全称 | Pre-B-Cell Colony-Enhancing Factor 1 |
| 反应种属 | Human |
| 应用 | Western Blot(0.2 µg/mL) Simple Western(2 µg/mL) Intracellular Staining by Flow Cytometry(0.25 µg/106cells) |
| 目标/特异性 | Detects human PBEF/Visfatin in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant rat PBEF is observed, and approximately 25% cross-reactivity with recombinant mouse PBEF is observed. |
| 使用方法 | Western Blot: 0.2 µg/mL Simple Western: 2 µg/mL Intracellular Staining by Flow Cytometry: 0.25 µg/106cells |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10135 (Human); 59027 (Mouse); 297508 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human PBEF/Visfatin isoform 1 Pro27-His491 Accession # P43490 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | PBEF (pre-B cell colony-enhancing factor; also visfatin and nicotinamide phosphoribosyltransferase) is a 52 kDa member of the NAPRTase family of molecules. It functions both intracellularly and extracellularly, where it participates in NAD synthesis and insulin receptor activation, respectively. Human PBEF is 491 amino acids in length and contains no signal sequence. There is at least one alternative splice form that shows a 5 aa substitution for the C-terminal 128 amino acids (aa 364-491). Over aa 27-491, human PBEF shares 96%, 97%, and 96% aa identity with mouse, porcine, and canine PBEF, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human PBEF/Visfatin by Western Blot. Western blot shows lysates of human heart tissue, 293T human embryonic kidney cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human PBEF/Visfatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4335) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PBEF/Visfatin at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of PBEF/Visfatin in PMA-treated Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells were treated for 24 hours with 50 ng/mL PMA then stained with Sheep Anti-Human PBEF/Visfatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4335, filled histogram) or control antibody (Catalog # 5‑001‑A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011) To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. | |
Detection of Human PBEF/Visfatin by Simple WesternTM. Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line, loaded at 0.2 mg/mL. A specific band was detected for PBEF/Visfatin at approximately 58 kDa (as indicated) using 2 µg/mL of Sheep Anti-Human PBEF/Visfatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4335) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
