| 货号 | AF4317-SP |
| 别名 | Alzheimer disease amyloid-associated protein; AMY; CLAC; CLACP; CLAC-P; COL25A1; Collagen 25; collagen alpha-1(XXV) chain; collagen, type XXV, alpha 1; Collagen-25; collagen-like Alzheimer amyloid plaque component; collagenous Alzheimer amyloid plaque component |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human Collagen XXV alpha 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human COL23A1 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 84570 (Human); 77018 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Collagen XXV alpha 1 Gly189-Lys654 Accession # NP_942014 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Collagen XXV (Collagen 25 alpha 1 chain; also CLAC-P) is a neuronal, 65‑75 kDa member of the Collagenous transmembrane (TM) subfamily of the Collagen protein family. It is synthesized as a 654 amino acid (aa) type II TM glycoprotein that contains a 33 aa cytoplasmic region (aa 1‑33) and a 600 aa extracellular domain (ECD) (aa 55‑654). The ECD contains seven Collagen-like domains. Furin cleavage between Arg112‑Glu113 generates a 70 kDa soluble fragment that homotrimerizes and binds to early A beta amyloid deposits. There are at least five multiple splice forms that impact the ECD. Amino acids 139‑146 and the seventh Collagen-like domain (aa 571‑630) are most often affected. Over aa 189‑654, human COL25A1 is 95% aa identical to mouse COL25A1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human Collagen XXV alpha 1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Collagen XXV alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4317) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Collagen XXV alpha 1 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
