Human IkB-epsilon Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human IkB-epsilon Affinity Purified Polyclonal Ab (25 UG)

货号： AF4300-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF4300-SP
别名	epsilon; ikappaBepsilon; I-kappa-B-epsilon; IkB-E; IKBENF-kappa-B inhibitor epsilon; IkB-epsilon; NF-kappa-BIE; NFKBIE; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor
全称	I-kappa-B-epsilon
反应种属	Human
应用	Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human IkB-epsilon in direct ELISAs. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse IkB-epsilon is observed and less than 1% cross-reactivity with recombinant human IkB-beta is observed.
使用方法	Immunohistochemistry: 5-15 µg/mL
来源	Sterile PBS to a final concentration of 0.2 mg/mL.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4794 (Human); 18037 (Mouse)
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human IkB-epsilon Ser141-Asp500 Accession # O00221
生物活性	Human
标记	Unconjugated
溶解方法	Sterile PBS to a final concentration of 0.2 mg/mL.
背景	NF kappa B inhibitor epsilon (IkB-epsilon ) inhibits the NF kappa B complex by binding and sequestering it in the cytoplasm. Upon stimulation, IkB-epsilon is phosphorylated on serine residues marking it for degradation by the ubiquitin pathway. Following IkB-epsilon degradation, the NF kappa B complex is able to translocate to the nucleus and activate transcription.
运输条件	Blue Ice
存放说明	4℃
参考文献	C-type Lectin Receptors ICC / IHC Images: IkB-epsilon IL-1 Family IL-17 Family NOD-like Receptors and the Inflammasome RIG-I-like Receptors Stem Cell Transcription Factor Regulators Toll-Like Receptors Transcription Factors and Regulators Transcriptional Co-Repressors

参考图片

IkB‑ epsilon in Human Astrocytoma. IkB‑ epsilon was detected in immersion fixed paraffin-embedded sections of human astrocytoma using Goat Anti-Human IkB‑ epsilon Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4300) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.