| 货号 | AF4299-SP |
| 别名 | alpha; I-kappa-B-alpha; IkappaBalphaNF-kappa-B inhibitor alpha; IKBAikB-alpha; IkB-alpha; MAD3; MAD-3; Major histocompatibility complex enhancer-binding protein MAD3; NFKBI; NFKBIA; nuclear factor of kappa light chain gene enhancer in B-cells; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor | 全称 | I-kappa-B-alpha |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects endogenous human and mouse IkB-alpha in Western blots. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4792 (Human); 18035 (Mouse); 25493 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse IkB-alpha Met1-Leu314 Accession # Q9Z1E3 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | NF kappa B inhibitor alpha (IkB-alpha ) inhibits the NF kappa B complex by binding and sequestering it in the cytoplasm. Upon stimulation, IkB-alpha is phosphorylated on serine residues marking it for degradation by the ubiquitin pathway. Following IkB-alpha degradation, the NF kappa B complex is able to translocate to the nucleus and activate transcription. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse IkB-alpha by Western Blot. Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line, DA3 mouse myeloma cell line, LNCaP human prostate cancer cell line, and Daudi human Burkitts lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Human/Mouse IkB-alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4299) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IkB-alpha at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
