Human LRPAP Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human LRPAP Affinity Purified Polyclonal Ab (25 UG)

货号： AF4296-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF4296-SP
别名	A2MRAP; A2MRAPMRAP; A2RAP; alpha-2-macroglobulin receptor-associated protein 1; alpha-2-macroglobulin receptor-associated protein; alpha-2-MRAP; HBP44; lipoprotein receptor associated protein; low density lipoprotein receptor-related protein associated protein 1; Low density lipoprotein receptor-related protein-associated protein 1; low density lipoprotein-related protein-associated protein 1(alpha-2-macroglobulin receptor-associated protein 1); LRPAP1; MGC138272; RAP
全称	Low Density Lipoprotein-Related Protein-associated Protein
反应种属	Human
应用	Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects human LRPAP in direct ELISAs and Western blots. In Western blots, approximately 30% cross‑reactivity with recombinant mouse LRPAP is observed.
使用方法	Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4043 (Human); 16976 (Mouse); 116565 (Rat)
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human LRPAP Tyr35-Leu357 Accession # P30533
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	LRPAP (LDL receptor-related protein-associated protein 1; also named RAP), is a ubiquitously expressed 39 kDa chaperone for LDL receptor family proteins (1, 2). Mature human LRPAP shares 77% amino acid sequence identity with mouse and rat LRPAP. It is organized into three domains of comparable length. Domains D2 and D3 interact with each other, while D1 is independent (3). The D1 domain contains a low affinity binding site for LRP, and the associated D2 and D3 domains bind LRP with high affinity (4). The majority of LRPAP is localized in the endoplasmic reticulum and Golgi (5). LRPAP prevents the premature interaction of LRP, LRP2/megalin, and VLDLR with their coexpressed ligands, thereby promoting proper receptor folding and export from the ER (6‑8). Protonation of conserved histidine residues within the D3 domain induces the separation of LRPAP and LRP in the relatively acidic Golgi (9). LRPAP, which contains a C-terminal HNEL motif, can then recycle to the ER (9). A minor amount of LRPAP remains associated with LRP and can modulate receptor activity on the cell surface (5). Exogenously applied LRPAP competitively inhibits LDL receptor family binding and uptake of activated alpha 2-macroglobulin, apoB100- or apoE-enriched LDL and VLDL particles, cholesteryl esters, and complexes of PAI-1 with either tPA or uPA (10‑14).
运输条件	Blue Ice
存放说明	4℃
参考文献	Strickland, D.K. et al. (1991) J. Biol. Chem. 266:13364. Bu, G. (2001) Int. Rev. Cytol. 209:79. Lazic, A. et al. (2003) Biochemistry 42:14913. Lazic, A. et al. (2006) Arch. Biochem. Biophys. 450:167. Bu, G. et al. (1994) J. Biol. Chem. 269:29874. Willnow, T.E. et al. (1996) EMBO J. 15:2632. Bu, G. and S. Rennke (1996) J. Biol. Chem. 271:22218. Obermoeller, L.M. et al. (1997) J. Biol. Chem. 272:10761. Lee, D. et al. (2006) Mol. Cell 22:423. Williams, S.E. et al. (1992) J. Biol. Chem. 267:9035. Medh, J.D. et al. (1995) J. Biol. Chem. 270:536. Herz, J. et al. (1991) J. Biol. Chem. 266:21232. Mokuno, H. et al. (1994) J. Biol. Chem. 269:13238. Orth, K. et al. (1992) Proc. Natl. Acad. Sci. 89:7422.

参考图片

LRPAP in A549 Human Cell Line. LRPAP was detected in immersion fixed A549 human lung carcinoma cell line using Goat Anti-Human LRPAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4296) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to Golgi and endoplasmic reticula. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.