| 货号 | AF4268-SP |
| 别名 | cAMP-dependent protein kinase catalytic subunit alpha; Cs; EC 2.7.11; EC 2.7.11.11; MGC102831; MGC48865; PKA C-alpha; Pkaca; PKCA1; PKCD; PRKACA; protein kinase A catalytic subunit; protein kinase, cAMP-dependent, catalytic, alpha | 全称 | cAMP-dependent Protein Kinase Catalytic Subunit alpha |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(2 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat PKA C alpha in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) PKA C beta or rhPKA Cδ is observed. |
| 使用方法 | Western Blot: 2 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5566 (Human); 18747 (Mouse); 25636 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human PKA C alpha Met1-Phe351 Accession # P17612 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The cAMP-dependent protein kinase (PKA) holoenzyme is composed of two regulatory and two catalytic subunits, designated PKA R and PKA C, respectively. Three catalytic subunit isoforms, C alpha, C beta, and C gamma, have been identified. Upon PKA R subunit binding to the second messenger cAMP, active PKA C subunits are released, initiating a phosphorylation cascade that regulates such cellular functions as metabolism, ion transport, and gene transcription. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse/Rat PKA C alpha by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL Goat Anti-Human/Mouse/Rat PKA C alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4268) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, Recombinant Human Active PKA C alpha (Catalog # 4268‑KS), Recombinant Human Active PKA C beta (Catalog # 4596‑KS), and recombinant human PKA C gamma (3 ng/lane) were included. A specific band for PKA C alpha was detected at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. |
