| 货号 | AF4256-SP |
| 别名 | DKFZp686O1631; GC-binding transcription factor Sp3; Sp3 transcription factor; specificity protein 3; SPR2; SPR-2; transcription factor Sp3 | 全称 | Sp3 Transcription Factor |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human Sp3 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6670 (Human); 20687 (Mouse); 367846 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Sp3 Asp53-Ser309 Accession # Q02447 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Sp3 is a member of the Sp1 family of C2H2-zinc-finger transcription factors. Sp3 binds to GT and GC boxes in promoters and can either stimulate or repress transcription. Knock out mice indicate that Sp3 is important particularly in erythroid and myeloid cell lineages. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
Detection of Human Sp3 by Western Blot. Western blot shows nuclear extracts of K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, and, Daudi human Burkitts lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Sp3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4256) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Sp3 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
