Human Glycoprotein V/CD42d Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human Glycoprotein V/CD42d Affinity Purified Polyclonal Ab (25 UG)

货号： AF4249-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF4249-SP
别名	platelet glycoprotein V; CD42d; glycoprotein V (platelet); GP5; GPV
反应种属	Human
应用	Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects human Glycoprotein V/CD42d in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human GPVI is observed.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2814 (Human); 14729 (Mouse); 25259 (Rat)
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human Glycoprotein V/CD42d Gln17-Gly523 Accession # P40197
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	GPV (platelet glycoprotein V; designated CD42d) is an 83 kDa type I transmembrane (TM) glycoprotein of the leucine-rich repeat (LRR) family (1, 2). It is expressed exclusively within the platelet / megakaryocyte lineage, where it noncovalently interacts with other platelet TM LRR proteins, GPIb alpha / beta and GPIX, at a ratio of one GPV to two of each other subunit (2). The GPI-V-IX complex tethers platelets to von Willebrand factor on the surface of injured endothelial cells. Absence of the complex results in Bernard-Soulier syndrome, a rare bleeding disorder (1‑3). The human GPV cDNA encodes a 560 amino acid (aa) protein with a 16 aa signal sequence, a 507 aa extracellular domain (ECD) containing 15 LRR, a 21 aa TM sequence, and a short (16 aa) cytoplasmic tail that binds calmodulin in resting, but not activated platelets. The human GPV ECD shares 70%, 71% and 81% aa identity with mouse, rat and equine GPV, respectively. GPV can form soluble fragments of 80 kDa by ADAM10 or ADAM17 cleavage after P507, or 69 kDa by thrombin cleavage after R476 (1, 4, 5). High circulating soluble GPV may be an indicator of platelet activation, but may also be caused by high doses of aspirin (6‑8). The function of GPV is not entirely clear. Deletion of GPV in mice does not produce any obvious change to surface expression or function of GPIb and GPIX, but surface expression of GPV requires GPIb (9, 10). Deletion studies also indicate that GPV may play a minor role in collagen adhesion, and may modify platelet aggregation in response to thrombin (3, 11‑15).
运输条件	Blue Ice
存放说明	4℃
参考文献	Lanza, F. et al. (1993) J. Biol. Chem. 268:20801. Hickey, M.J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:8327. Ozaki, Y. et al. (2005) J. Thromb. Haemost. 3:1745. Rabie, T. et al. (2005) J. Biol. Chem. 280:14462. Gardiner, E.E. et al. (2007) J. Thromb. Haemost. 5:1530. Wolff, V. et al. (2005) Stroke 36:e17. Javela, K. et al. (2005) Transfusion 45:1504. Aktas, B. et al. (2005) J. Biol. Chem. 280:39716. Kahn, M.L. (1999) Blood 94:4112. Strassel, C. et al. (2004) Eur. J. Biochem. 271:3671. Nonne, C. et al. (2008) J. Thromb. Haemost. 6:210. Moog, S. et al. (2001) Blood 98:1038. Ramakrishnan, V. et al. (1999) Proc. Natl. Acad. Sci. USA 96:13336. Ni, H. et al. (2001) Blood 98:368. Andrews, R.K. et al. (2001) Blood 98:681.

参考图片

Detection of Human GPV in Human Whole Blood Platelets by Flow Cytometry.

Human whole blood platelets were stained with Sheep Anti-Human GPV Polyclonal Antibody (Catalog # AF4249) followed by NL637-conjugated anti-sheep antibody (Catalog # NL011) and Human CD41 FITC-conjugated Monoclonal Antibody.Quadrant markers were set based on control antibody staining (Catalog # 5‑001‑A).