| 货号 | AF4244-SP |
| 别名 | FLJ10160; insulin gene enhancer protein ISL-2; ISL LIM homeobox 2; ISL2 transcription factor, LIM/homeodomain, (islet-2); ISL2; islet-2 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Islet-2 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 64843 (Human); 104360 (Mouse); 57233 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Islet-2 Met1-Thr359 Accession # Q96A47 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Islet-2 is a member of the LIM homeodomain transcription factors and its expression pattern defines a subclass of motor neurons. Islet-2 contains two amino terminal LIM domains and a carboxy terminal homeodomain. Human Islet-2 shares 93% similarity with mouse Islet-2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Islet‑2 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Human Islet‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4244) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Islet‑2 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
