| 货号 | AF4238-SP |
| 别名 | KIAA1329VPS10 domain receptor protein; sortilin-related VPS10 domain containing receptor 2; VPS10 domain-containing receptor SorCS2 | 全称 | Sortilin-related VPS10 Domain Containing Receptor 2 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human SorCS2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant mouse (rm) SorCS2 is observed and less than 1% cross-reactivity with recombinant human (rh) SorCS1 and rhSorCS3 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 57537 (Human); 81840 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | NS0-derived recombinant human SorCS2 Ser70-Gly1078 Accession # Q96PQ0 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | SorCS2 is a type I transmembrane glycoprotein receptor that belongs to the mammalian Vps10p (vacuolar protein-sorting 10 protein) family (1, 2). Family receptors include sortilin, SorLA, and three SorCS proteins. All SorCS proteins are predominantly expressed in the brain, especially during development, but vary in other locations (1‑3). SorCS2 mRNA has also been found in the kidney, lung, testis and heart (1, 2). SorCS2 presumably mediates endocytosis, but its ligands are not well‑defined. However, the other SorCS proteins have been shown to bind NGF and PDGF-BB (4‑6). Human SorCS2 is synthesized as a 1159 amino acid (aa) prepro form with a 50 aa signal sequence and a potential furin-type proteolytic processing site at aa 119. This would produce a mature SorCS2 protein of 1040 aa with a 959 aa extracellular/lumenal domain (ECD), a 21 aa transmembrane domain and a 60 aa cytoplasmic domain. The ECD contains an imperfect leucine-rich repeat (LRR) and a Vps10p domain (2). Within the ECD, human SorCS2 shares 89%, 88%, 88% and 79% aa identity with mouse, rat, equine and canine SorCS2, respectively. It also shares 46% aa identity with the ECD of both SorCS1 and SorCS3. Unlike other SorCS, shedding of the SorCS2 ECD occurs very slowly and is mainly independent of the metalloproteinase TACE/ADAM17 (4). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human SorCS2 by Western Blot. Western blot shows lysates of human kidney (medulla) tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human SorCS2 Antigen Affinity-purified Antibody (Catalog # AF4238) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SorCS2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
SorCS2 in Human Brain. SorCS2 was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Sheep Anti-Human SorCS2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4238) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurons and their processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
