Human Integrin alpha 11 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human Integrin alpha 11 Affinity Purified Polyclonal Ab (25 UG)

货号： AF4235-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF4235-SP
别名	HsT18964; integrin alpha-11; integrin, alpha 11; ITGA11
反应种属	Human
应用	Western Blot(1 µg/mL)
目标/特异性	Detects human Integrin alpha 11 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Integrin alpha 11 is observed and less than 1% cross-reactivity with recombinant human (rh) Integrin alpha 1 and rhIntegrin alpha 2 is observed.
使用方法	Western Blot: 1 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	22801 (Human); 319480 (Mouse); 315744 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Tenascin-X promotes epithelial-to-mesenchymal transition by activating latent TGF-beta. Authors: Alcaraz L, Exposito J, Chuvin N, Pommier R, Cluzel C, Martel S, Sentis S, Bartholin L, Lethias C, Valcourt U J Cell Biol, 2014;205(3):409-28. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Chinese hamster ovary cell line CHO-derived recombinant human Integrin alpha 11 Phe23-Pro1141 (Leu524Arg) Accession # NP_001004439
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Integrin alpha 11 (ITGA11) is a 155-160 kDa member of the integrin alpha chain family of molecules. It preferentially forms a cell surface heterodimer with beta 1 integrin. In particular, it is further classified as a collagen-binding group member, showing a preference for binding to collagen I and II. ITGA11 is expressed by embryonic mesenchymal cells in and around areas that incorporate, or utilize collagen during development. In adult tissue, fibroblasts express ITGA11, and collagen:ITGA11 interaction likely contributes to wound contraction and closure. Mature human ITGA11 is an 1167 amino acid (aa) type I transmembrane glycoprotein. It contains a large 1120 aa extracellular domain (ECD) (aa 23-1142) plus a short 24 aa cytoplasmic region. The ECD shows 7 x 60 aa FG-Gap repeats that generate beta -propellers (aa 24-650) with an intervening I domain that binds collagen (aa 154-345). Over aa 23-1142, human ITGA11 shares 90% aa identity with mouse ITGA11.
运输条件	Blue Ice
存放说明	4℃
参考文献	Actin Stress Fibers and Focal Adhesions During EMT Adhesion Molecules HGF/SF Activation of Integrins ICC / IHC Images: Integrin alpha 11 Integrin Ligands at a Glance (J. Cell. Sci.) Integrin Subunit Interactions Integrins and Associated Molecules

参考图片

Detection of Human Integrin alpha 11 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Integrin alpha 11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4235) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Integrin alpha 11 at approximately 155 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.