| 货号 | AF4197-SP |
| 别名 | CNK; CNKpolo-like kinase 3 (Drosophila); cytokine-inducible kinase; Cytokine-inducible serine/threonine-protein kinase; EC 2.7.11; FNK; FNKEC 2.7.11.21; polo-like kinase 3PLK-3; PRK; PRKFGF-inducible kinase; Proliferation-related kinase; serine/threonine-protein kinase PLK3 | 全称 | Polo-like Kinase 3 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human PLK3 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1263 (Human); 12795 (Mouse); 58936 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human PLK3 Pro320-Ala520 Accession # Q9H4B4.2 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Polo-like kinases are critical regulators of cell cycle progression, cytokinesis, mitosis, and the genotoxic stress response. Four distinct members have been identified to date in mammalian cells: PLK1, PLK2, PLK3, and PLK4. PLK3 (polo-like kinase 3) plays an important role in cell cycle regulation by modulating microtubule dynamics and centrosomal function. Although protein levels are unchanged throughout the cell cycle, kinase activity peaks during late S and G2 phases. Many key cell cycle regulators are phosphorylated by PLK3 including p53 on Ser20, Cdc25C on Ser216, and the major subunit of DNA polymerase δ, p125 on Ser60. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human PLK3 by Western Blot. Western blot shows nuclear extracts of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human PLK3 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4197) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PLK3 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
