| 货号 | AF4195-SP |
| 别名 | CARFCDKN2A-interacting protein; CDKN2A interacting protein; CDKN2AIP; collaborates/cooperates with ARF (alternate reading frame) protein; Collaborator of ARF; FLJ20036 | 全称 | Collaborates/Cooperates With ARF [Alternate Reading Frame] Protein |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human CARF in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 55602 (Human); 70925 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human CARF Ser379-Leu579 Accession # Q9NXV6 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | CARF (collaborator of ARF) is a component of the ARF-MDM2-p53 pathway. CARF interacts with ARF in the nucleolus accentuating ARF-mediated p53 stabilization and activation. Data also suggests that in the absence of ARF, CARF interacts directly with p53 in the nucleoplasm resulting in a moderate stabilization and activation of p53. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human CARF by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line, SK-Mel-28 human malignant melanoma cell line, and U2OS human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Human CARF Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4195) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CARF at approximately 61 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1. |
