| 货号 | AF4185-SP |
| 别名 | BHLHC11; Class C basic helix-loop-helix protein 11; MAD2; MAX dimerization protein 2; MAX interacting protein 1; MAX interactor 1bHLHc11MAD2; max-interacting protein 1; Max-related transcription factor; MGC43220; MXD2; MXI; MXI-WR | 全称 | MAX Interacting Protein 1 |
| 反应种属 | Human |
| 应用 | Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Mxi1 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) MAD1, rhMAD3, and rhMAD4 is observed. |
| 使用方法 | Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4601 (Human); 17859 (Mouse); 25701 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Mxi1 Ala81-Ser228 Accession # P50539 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Mxi-1 (Max interactor 1) is a 26 kDa member of the bHLH-leucine zipper family, Myc superfamily of transcription factors. It is a widely expressed transcriptional repressor that heterodimerizes with Max and blocks Max-Myc gene activation. Human Mxi-1 is 228 amino acids (aa) in length. It contains a DNA binding motif (aa 69‑80) and a bHLH domain (aa 81‑120). Multiple splice variants of Mxi-1 appear to exist. They may incorporate an alternate start site at Met37, a 92 aa substitution for the first 26 N-terminal amino acids, or a deletion of the C-terminal 59 or 74 amino acids. Over aa 81‑228, human Mxi-1 is 95% aa identical to mouse Mxi-1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Mxi1 in LNCaP Human Cell Line. Mxi1 was detected in immersion fixed LNCaP human prostate cancer cell line using Goat Anti-Human Mxi1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4185) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
