| 货号 | AF4179-SP |
| 别名 | Chemokine-like protein TAFA-2; DKFZp761E1217; DKFZp781P0552; FAM19A2; family with sequence similarity 19 (chemokine (C-C motif)-like), member A2; MGC42403; TAFA2; TAFA-2 | 全称 | Family with Sequence Similarity 19, Member A2 |
| 反应种属 | Human |
| 应用 | Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human TAFA2/FAM19A2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human (rh) TAFA3 and rhTAFA4 is observed, and less than 2% cross-reactivity with rhTAFA5 is observed. |
| 使用方法 | Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize the the enhancemment of neurite outgrowth of cortical neurons from E16-E18 rat embryos induced by TAFA-2. The Neutralization Dose (ND50) is typically 5 µg/mL in the presence of 10 µg/mL Recombinant Human TAFA-2. |
| 来源 | Polyclonal Sheep IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 338811 (Human); 268354 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human TAFA2/FAM19A2 Ala31-His131 Accession # Q8N3H0 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | TAFA2 (also FAM19A2) is a secreted, 11 kDa member of the FAM19/TAFA family of chemokine-like proteins (1). It is synthesized as a 131 amino acid (aa) precursor that contains a 30 aa signal sequence and a 101 aa mature chain. Like other members of the FAM19/TAFA family, with the exception of TAFA5, mature TAFA1 contains 10 regularly spaced cysteine residues that follow the pattern CX7CCX13CXCX14CX11CX4CX5CX10C, where C represents a conserved cysteine residue and X represents any noncysteine amino acid (1). Human TAFA2 is 97% aa identical to mouse TAFA2 (1). TAFA2 expression can be detected in the central nervous system (CNS), colon, heart, lung, spleen, kidney, and thymus, but its expression in the CNS is 50- to 1000-fold higher than in other tissues (1). Within the CNS, TAFA2 expression is highest in the occipital and frontal cortex (3- to 10-fold more abundantly expressed than in other cortical regions) and medulla (1). The biological functions of TAFA family members remain to be determined, but there are a few tentative hypotheses. First, TAFAs may modulate immune responses in the CNS by functioning as brain-specific chemokines, and may act with other chemokines to optimize the recruitment and activity of immune cells in the CNS (1). Second, TAFAs may represent a novel class of neurokines that act as regulators of immune nervous cells (1 - 2). Finally, TAFAs may control axonal sprouting following brain injury (1). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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TAFA2/FAM19A2 in Human Brain. TAFA2/FAM19A2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Human TAFA2/FAM19A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4179) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
