| 货号 | AF4177-SP |
| 别名 | PRKAR1B; RIbeta | 全称 | cAMP-dependent Protein Kinase Regulatory Type I beta |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) Simple Western(10 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat PKA RI beta in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) PKA RI alpha or rhPKA RII beta is observed; cross-reactivity with PKA RII alpha is unknown. |
| 使用方法 | Western Blot: 1 µg/mL Simple Western: 10 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5575 (Human); 19085 (Mouse); 25521 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. microRNA-208a in an early stage myocardial infarction rat model and the effect on cAMP-PKA signaling pathway | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human PKA RI beta Met1-Val381 Accession # P31321 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The cAMP-dependent protein kinase (PKA) holoenzyme is composed of two regulatory and two catalytic subunits, designated PKA R and PKA C, respectively. PKA R subunits exist as two classes, RI and RII, with each class containing two isoforms, alpha and beta. Upon PKA R subunit binding to the second messenger cAMP, active PKA C subunits are released, initiating a phosphorylation cascade that regulates such cellular functions as metabolism, ion transport, and gene transcription. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human PKA RI beta by Western Blot. Western blot shows lysates of MDA-MB-468 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, SW480 human colorectal adenocarcinoma cell line, Raji human Burkitts lymphoma cell line, and Ramos human Burkitts lymphoma cell line. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse/Rat PKA RI beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4177) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for PKA RI beta was detected at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of Human PKA RI beta by Western Blot. Western blot shows recombinant human PKA RI alpha, PKA RI beta, and PKA RII beta (5 ng/lane). PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/ | |
Detection of Human PKA RI beta by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for PKA RI beta at approximately 56 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human/Mouse/Rat PKA RI beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4177) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
