| 货号 | AF4176-SP |
| 别名 | calcium/calmodulin-dependent protein kinase (CaM kinase) II delta; calcium/calmodulin-dependent protein kinase II delta; calcium/calmodulin-dependent protein kinase type II delta chain; calcium/calmodulin-dependent protein kinase type II subunit delta; CaM kinase II delta subunit; CaM kinase II subunit delta; CAMK2D; CAMKD; CaMK-II delta subunit; CaMK-II subunit delta; CaM-kinase II delta chain; DKFZp686G23119; DKFZp686I2288; EC 2.7.11; EC 2.7.11.17; MGC44911 | 全称 | Calcium/calmodulin-dependent Protein Kinase II delta Subunit |
| 反应种属 | Human |
| 应用 | Western Blot(2 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human CaM Kinase IIδ isoforms. No cross-reactivity with recombinant human CaM Kinase II alpha, CaM Kinase II beta, or CaM Kinase II gamma is observed. |
| 使用方法 | Western Blot: 2 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 817 (Human); 108058 (Mouse); 24246 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human CaM Kinase IIδ Ala402-Lys478 Accession # Q13557 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Calcium/calmodulin-dependent protein kinase type II (CaMKII) belongs to a family of multifunctional serine/threonine kinases activated in response to increases in intracellular calcium. There are 4 CaMKII isozymes, alpha, beta, gamma, and δ, and each can yield several isoforms through alternative splicing. Each CaMKII protein assembles into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. CaMKIIδ expression is ubiquitous, and has been identified as the predominant isozyme in the heart, where it is implicated in the development of cardiac hypertrophy and heart failure. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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CaM Kinase IIδ in Human Brain. CaM Kinase IIδ was detected in immersion fixed paraffin-embedded sections of human brain (cerebral cortex) using 15 µg/mL Sheep Anti-Human CaM Kinase IIδ Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4176) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
Detection of Human CaM Kinase IIδ by Western Blot. Western blot shows lysates of SW480 human colorectal adenocarcinoma cell line, K562 human chronic myelogenous leukemia cell line, MDA‑MB‑468 human breast cancer cell line, and SK‑Mel‑28 human malignant melanoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human CaM Kinase IIδ Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4176) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CaM Kinase IIδ at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
