Human IGSF4C/SynCAM4 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human IGSF4C/SynCAM4 Affinity Purified Polyclonal Ab (25 UG)

货号： AF4164-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF4164-SP
别名	CADM4; cell adhesion molecule 4; IgSF4C; IGSF4Cnectin-like 4; Immunoglobulin superfamily member 4C; immunoglobulin superfamily, member 4C; Necl-4; NECL4synCAM4; Nectin-like protein 4; SynCAM4; TSLC1-like 2; TSLC1-like protein 2; TSLL2; TSLL2SynCAM4
全称	Immunoglobulin Superfamily, Member 4B
反应种属	Human
应用	Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects human IGSF4C/SynCAM4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross‑reactivity with recombinant human (rh) IGSF4B is observed and less than 1% cross‑reactivity with rhIGSF4D is observed.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	199731 (Human); 260299 (Mouse)
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human IGSF4C/SynCAM4 Gln25-Tyr323 Accession # Q8NFZ8
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	IGSF4C is an immunoglobulin superfamily member that is also known as Nectin-like protein 4 (Necl-4), synaptic cell adhesion molecule 4 (SynCAM4), or tumor suppressor in lung cancer-like 2 (TSLL-2) (1, 2). The four IGSF4 proteins, designated A, B, C and D, are type I transmembrane glycoproteins expressed mainly in neurons, but also in lung, kidney, bladder, prostate and testis (1‑5). Their extracellular domains (ECD) share 35‑50% amino acid (aa) identity and each contain one V‑type Ig-like and two C2-type Ig-like domains. These domains are responsible for Ca⁺⁺-independent homophilic and heterophilic interactions. The 388 aa human IGSF4C contains a 20 aa signal sequence, a 304 aa ECD that shares 98‑99% amino acid identity with mouse, rat, canine and bovine IGSF4C, a 21 aa transmembrane sequence, and a 43 aa cytoplasmic domain. The apparent size of mouse or human IGSF4C may be variably reported as 48‑67 kDa, probably due to differences in glycosylation (2, 5, 8). In the peripheral nervous system, IGSF4C is expressed on Schwann cells, and its internodal interaction with IGSF4A (Necl-1, SynCAM-3) on axons is critical for adhesion and myelination (6‑8). In the brain, all IGSF4 family members are expressed at high levels concurrent with synapse formation (4). In the cerebellum, IGSF4C is expressed on Purkinje cells, with complementary expression of IGSF4 on granule cells (4). Heterophilic interaction with IGSF4D (Necl-3, SynCAM2) has also been identified, but homophilic interaction is unlikely (4, 6). IGSF4C is also proposed as a tumor suppressor that is downregulated in many prostate cancers and gliomas (1, 5).
运输条件	Blue Ice
存放说明	4℃
参考文献	Fukuhara, H. et al. (2001) Oncogene 20:5401. Biederer, T. et al. (2006) Genomics 87:139. Takai, Y. et al. (2008) Nat. Rev. Mol. Cell Biol. 9:603. Thomas, L. A. et al. (2008) J. Comp. Neurol. 510:47. Williams, Y. N. et al. (2005) Oncogene 25:1446. Fogel, A. I. et al. (2007) J. Neurosci. 27:12516. Spiegel, I. et al. (2007) Nat. Neurosci. 10:861. Maurel, P. et al. (2007) J. Cell Biol. 178:861.

参考图片

Detection of IGSF4C/SynCAM4 in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Goat Anti-Human IGSF4C/SynCAM4 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4164, filled histogram) or control antibody (Catalog # AB‑108‑C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).