| 货号 | AF4149-SP |
| 别名 | annexin A13; Annexin XIII; Annexin-13; ANX13; ANX13annexin, intestine-specific; ANXA13; Intestine-specific annexin; ISA; ISAannexin-13; MGC150460 |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects endogenous human and mouse Annexin A13 in Western blots. In Western blots, this antibody did not cross-react with recombinant human Annexin A1, A2, A3, A4, A5, A6, A7, A8, A9, A10, or A11. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 312 (Human); 69787 (Mouse); 362915 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The tolerogenic function of annexins on apoptotic cells is mediated by the annexin core domain. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Annexin A13 Met1-His316 Accession # P27216 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Annexin A13 (ANXA13), also known as Annexin XIII and Intestine-Specific Annexin, is a member of the Annexin protein family. Two isoforms have been identified resulting from alternate splicing. Isoform A consists of 316 amino acids and has a predicted molecular weight of 36 kDa whereas isoform B is composed of 357 amino acids and has a predicted molecular weight of 40 kDa. Annexins are a family of Calcium-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane. The Annexin’s have a molecular weight of approximately 35 to 40 kDa and consist of a unique amino terminal domain followed by a homologous C-terminal core domain containing the calcium-dependent phospholipid-binding sites. The C terminal domain is comprised of four 60‑70 amino acid repeats, known as annexin repeats or an endonexin fold (Annexin A6 contains 8 annexin repeats). The four annexin repeats form a highly alpha -helical, tightly packed disc known as the annexin domain, which binds to phospholipids in the membrane in a calcium-dependent manner. Members of the annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth, and intracellular signal transduction pathways. The specific function of Annexin A13 has not been determined but it is associated with the plasma membrane of undifferentiated, proliferating endothelial cells and differentiated villus enterocytes. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse Annexin A13 by Western Blot. Western blot shows lysates of human and mouse small intestine tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse Annexin A13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4149) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Annexin A13 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
