| 货号 | AF4148-SP |
| 别名 | Heat shock 70 kDa protein 8; heat shock 70kD protein 8; heat shock 70kDa protein 8; heat shock cognate 71 kDa protein; heat shock cognate protein 54; heat shock cognate protein, 71-kDa; HSC54; HSC70; HSC70heat shock 70kd protein 10; HSC71; HSP71; HSP73; HSP73constitutive heat shock protein 70; HSPA10; LAP1; lipopolysaccharide-associated protein 1; LPS-associated protein 1; MGC131511; MGC29929; NIP71; N-myristoyltransferase inhibitor protein 71 | 全称 | Heat Shock 70 kDa Protein 8 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(3-15 µg/mL) |
| 目标/特异性 | Detects human, mouse and rat HSPA8/HSC71, Isoform 1 in Western blots. In Western blots, this antibody shows no cross‑reactivity with recombinant human HSPA1A/HSP70, HSPA2, HSPA6, GRP75 or GRP78. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 3-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3312 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human HSPA8 Asp534-Gly615 Accession # P11142 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The 70 kDa heat shock proteins (HSP70s) are a highly conserved family of stress response proteins. The HSP70 family of proteins contains both heat/stress inducible and constitutively expressed members known as heat shock cognate proteins. Heat Shock 70 kDa Protein 8 (HSPA8), also known as Heat Shock Cognate Protein 71 (HSC71), HSC70, HSP73, HSPA10 and LPS-associated protein 1 (LAP1), is a 646 amino acid (aa) heat shock cognate protein. Many HSPs function as molecular chaperones, facilitating the folding of other cellular proteins. HSPA8 plays an important role in cells by transiently associating with nascent polypeptides to facilitate correct folding. HSPA8 is a ubiquitously expressed protein and rapidly translocates from the cytoplasm to the nucleus in response to heat shock. Two alternatively spliced variants have been identified. This antibody detects isoform 1 but not isoform 2 (HSC54). Human HSPA8 shares greater than 99% identity with mouse and rat HSPA8. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse/Rat HSPA8/HSC71 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat HSPA8/HSC71 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4148) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for HSPA8/HSC71 at approximately 71 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2. | |
HSPA8/HSC71 in Mouse Intestine. HSPA8/HSC71 was detected in immersion fixed frozen sections of mouse intestine using Goat Anti-Human/Mouse/Rat HSPA8/HSC71 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4148) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells in intestinal glands. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. | |
HSPA8/HSC71 in Rat Intestine. HSPA8/HSC71 was detected in immersion fixed frozen sections of rat intestine using Goat Anti-Human/Mouse/Rat HSPA8/HSC71 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4148) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells in intestinal glands. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. | |
HSPA8/HSC71 in Human Kidney. HSPA8/HSC71 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human/Mouse/Rat HSPA8/HSC71 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4148) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
