| 货号 | AF4109-SP |
| 别名 | protein Wnt-6; wingless-type MMTV integration site family, member 6 | 全称 | Wingless-type MMTV Integration Site Family, Member 6 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Wnt-6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse (rm) Wnt-4, rmWnt-8a, and rmWnt-9b is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7475 (Human); 22420 (Mouse); 316526 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Wnt‑6 Met35-Glu75 and Gln238-Arg299 Accession # Q9Y6F9 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Human Wnt-6 is a 46 kDa, secreted, glycoprotein of the Wnt family. Human Wnt-6 is synthesized as a 365 amino acid (aa) precursor that contains a 341 aa mature region. The mature molecule contains 24 cysteines and one potential N-linked glycosylation site. Wnt-6 is produced by mammary fibroblasts and ductal epithelium, intestinal crypt cells, and limb bud ectoderm. Over the regions used as immunogen, the aa sequences of human, mouse, and canine Wnt-6 are identical. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Wnt-6 in Human Colon Cancer Tissue. Wnt-6 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Sheep Anti-Human Wnt-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4190) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
