| 货号 | AF4108-SP |
| 别名 | cancer susceptibility; FLJ25530; glial cell adhesion molecule; GlialCAM; hepaCAM; hepatic and glial cell adhesion molecule; hepatocyte cell adhesion molecule; Protein hepaCAM | 全称 | Hepatocyte Cell Adhesion Molecule |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human HepaCAM in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 220296 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human HepaCAM Val34-Tyr242 Accession # Q14CZ8 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Hepatocyte Cell Adhesion Molecule (HepaCAM) is a 416 amino acid type I transmembrane glycoprotein that belongs to the immunoglobin superfamily. HepaCAM forms cis-homodimers on the cell surface and may be involved in modulating cell-matrix interactions, likely by promoting cell spreading and motility. Human HepaCAM runs as a 48-72 kDa protein depending on glycosylation. It contains a 206 amino acid extracellular domain and a 152 amino acid cytoplasmic domain. Over the extracellular region used as immunogen (aa 34-242), human HepaCAM is 99% and 98% identical to mouse and canine HepaCAM proteins, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human HepaCAM by Western Blot. Western blot shows lysates of human brain (motor cortex) tissue and human brain (hippocampus) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human HepaCAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4108) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for HepaCAM at approximately 45-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
