| 货号 | AF4080-SP |
| 别名 | BBF-2 Homolog; cAMP responsive element binding protein 3-like 1; cAMP-responsive element-binding protein 3-like protein 1; CREB3L1; OASIScyclic AMP-responsive element-binding protein 3-like protein 1; old astrocyte specifically induced substance; Old astrocyte specifically-induced substance | 全称 | Old Astrocyte Specifically Induced Substance |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human OASIS/CREB3L1 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 90993 (Human); 26427 (Mouse); 362165 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Transcription Factor CREB3L1 Regulates Endoplasmic Reticulum Stress Response Genes in the Osmotically Challenged Rat Hypothalamus. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human OASIS/CREB3L1 Met1-Asp111 Accession # Q96BA8 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | OASIS (old astrocyte specifically-induced substance) is a 57‑60 kDa member of the CREB/ATF family of transcription factors. Human OASIS is 519 amino acids (aa) in length. It is an ER transmembrane protein with a cytosolic N-terminus (aa 1‑374) and a luminal C‑terminus. The N-terminus contains one b-Zip region (aa 292‑353). Misfolded proteins in the ER induce proteolytic cleavage of OASIS. The released 66 kDa N-terminus translocates to the nucleus to activate stress response genes. There is one alternate splice form that shows a deletion of aa 112‑198. Over aa 1‑111, human OASIS shares 96% aa sequence identity with mouse OASIS. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human OASIS/CREB3L1 by Western Blot. Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human OASIS/CREB3L1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4080) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for OASIS/CREB3L1 at approximately 57 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
