| 货号 | AF4069-SP |
| 别名 | FGFR signalling adaptor; FGFR-signaling adaptor SNT; fibroblast growth factor receptor substrate 2; FRS2A; FRS2alpha; SNT; SNT1; SNT-1FGFR substrate 2; Suc1-associated neurotrophic factor target 1 | 全称 | Fibroblast Growth Factor Receptor Substrate 2 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human FRS2 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10818 (Human); 327826 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. In Vitro and In Vivo Activity of Lucitanib in FGFR1/2 Amplified or Mutated Cancer Models | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human FRS2 Asn121-Asn449 Accession # Q8WU20 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | FRS2 (FGFR substrate 2; also known as SNT and FRS2-alpha ) is a 70‑90 kDa member of the FRS family of lipid-anchored docking proteins. It is an intermediary between FGF and TRK receptors and their Ras/MAPK signaling cascades. Human FRS2 is 512 amino acids (aa) in length. It contains a membrane-anchoring myristoylation signal (aa 1‑6), a PTB domain that interacts with FGF and NGF receptors (aa 13‑115), and a C-terminal Tyr-rich region that serves as a docking site for Grb2 and Shp2 (aa 196‑471). One splice form shows a deletion of the C-terminal four amino acids. Over aa 121‑449, human FRS2 is 99% and 94% aa identical to canine and mouse FRS2, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
FRS2 in Human Liver and Liver Cancer Tissue. FRS2 was detected in immersion fixed paraffin-embedded sections of human normal liver and liver cancer tissue using Sheep Anti-Human FRS2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4069) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cell cytoplasm and cell membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
