Human Integrin beta 4/CD104 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human Integrin beta 4/CD104 Affinity Purified Polyclonal Ab (25 UG)

货号： AF4060-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF4060-SP
别名	CD104 antigen; CD104; GP150; integrin beta-4 subunit; integrin beta-4; integrin, beta 4; ITGB4
反应种属	Human
应用	Western Blot(0.1 µg/mL) Flow Cytometry(0.25 µg/10⁶cells) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects human Integrin beta 4/CD104 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross‑reactivity with recombinant mouse Integrin beta 4 is observed and less than 1% cross-reactivity with recombinant human (rh) Integrin beta 2, rhIntegrin beta 3, and rhIntegrin beta 5 is observed.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 0.25 µg/10⁶cells Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3691 (Human); 192897 (Mouse); 25724 (Rat)
纯化方式	Antigen Affinity-purified
免疫原	Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 4/CD104 Asn28-Ser710 Accession # P16144
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Integrin beta 4 (also known as GP150 and CD104) is a 150‑200 kDa member of the Integrin beta family. It forms noncovalent heterodimers with Integrin alpha 6 and participates in the formation of epithelial hemidesmosomes. Human Integrin beta 4 is a type I transmembrane glycoprotein that is 1795 amino acids (aa) in length. It contains a 683 aa extracellular domain (ECD) (aa 28‑710) and a large 1089 aa cytoplasmic region. Two ECD alternate splice forms may exist. One shows an 84 aa substitution for the 84 aa between aa 621‑704. A second shows a 144 aa substitution for the first 704 amino acids. Human Integrin beta 4 ECD shares 88% sequence identity with mouse Integrin beta 4 ECD.
运输条件	Blue Ice
存放说明	4℃
参考文献	Actin Stress Fibers and Focal Adhesions During EMT Adhesion Molecules Endothelial Cell Markers and Adhesion Molecules in Angiogenesis Epithelial Cell Polarity HGF/SF Activation of Integrins Human CD Marker Products Integrin Ligands at a Glance (J. Cell. Sci.) Integrin Subunit Interactions Integrins and Associated Molecules

参考图片

Integrin beta 4/CD104 in A431 Human Cell Line. Integrin beta 4/CD104 was detected in immersion fixed A431 human epithelial carcinoma cell line using Sheep Anti-Human Integrin beta 4/CD104 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4060) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.