| 货号 | AF4050-SP |
| 别名 | BHLHE38; bHLHe38myc-related gene from lung cancer; Class E basic helix-loop-helix protein 38; L-Myc; LMYCl-myc-1 proto-oncogene; MYCL; protein L-Myc-1; v-myc avian myelocytomatosis viral oncogene homolog 1, lung carcinoma derived; v-myc myelocytomatosis viral oncogene homolog 1, lung carcinoma derived (avian) | 全称 | v-Myc Myelocytomatosis Viral Oncogene Homolog 1, Lung Carcinoma Derived |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human and mouse MYCL1/L‑Myc in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4610 (Human); 16918 (Mouse); 298506 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Merkel Cell Polyomavirus Small T Antigen Promotes Pro-Glycolytic Metabolic Perturbations Required for Transformation | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human MYCL1/L‑Myc Gly16-Asn139 Accession # P12524 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | MYCL1 is a member of the MYC family that was isolated from small cell lung carcinoma. Like the other members of the MYC family, MYCL1 is a proto-oncogene transcription factor belonging to the helix-loop-helix basic leucine zipper (HLH bzip) family. With its heterodimeric partners, MYCL1 binds to the DNA consensus site 5’ CACGTG 3’. MYCL1 is implicated in controlling a wide range of cellular processes from cellular proliferation to apoptosis. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse MYCL1/ L‑Myc by Western Blot. Western blot shows nuclear extracts of HeLa human cervical epithelial carcinoma cell line, A549 human lung carcinoma cell line, JEG‑3 human epithelial choriocarcinoma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse MYCL1/L‑Myc Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4050) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for MYCL1/L‑Myc at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. | |
MYCL1/L‑Myc in HeLa Human Cell Line. MYCL1/L‑Myc was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse MYCL1/L‑Myc Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4050) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, blue panel). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
MYCL1/L‑Myc in NIH3T3 Mouse Cell Line. MYCL1/L‑Myc was detected in immersion fixed NIH3T3 mouse embryonic fibroblast cell line using Goat Anti-Human/Mouse MYCL1/L‑Myc Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4050) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
