Human Smad2 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human Smad2 Affinity Purified Polyclonal Ab (25 UG)

货号： AF4037-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF4037-SP
别名	hMAD-2; hSMAD2; JV18; JV18-1; JV18-1SMAD, mothers against DPP homolog 2 (Drosophila); MAD homolog 2; Mad protein homolog; Mad, mothers against decapentaplegic homolog 2; MADH2; MADH2MGC34440; MADR2; MADR2MGC22139; Mad-related protein 2; mother against DPP homolog 2; mothers against decapentaplegic homolog 2; Mothers against DPP homolog 2; Sma- and Mad-related protein 2; SMAD 2; SMAD family member 2MAD, mothers against decapentaplegic homolog 2 (Drosophila); SMAD, mothers against DPP homolog 2; Smad2; Smox
全称	Mothers Against DPP Homolog 2
反应种属	Human
应用	Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human Smad2 in direct ELISAs.
使用方法	Immunohistochemistry: 5-15 µg/mL
来源	Sterile PBS to a final concentration of 0.2 mg/mL.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4087 (Human); 17126 (Mouse); 29357 (Rat); 31738 (Drosophila)
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human Smad2 Lys20-Thr108 Accession # Q15796
生物活性	Human
标记	Unconjugated
溶解方法	Sterile PBS to a final concentration of 0.2 mg/mL.
背景	SMAD2 (Sma and MAD-related protein 2; also MAD2, MADR2 and MADH2) is a 58-62 kDa member of the dwarfin/SMAD family of proteins. It is widely expressed, particularly in striated muscle, and exists constitutively in the cytoplasm. SMAD2 is a downstream mediator of TGF-beta and activin signaling. In particular, SMAD2 is a nonphosphorylated monomer in unstimulated cells. Upon ALK-4, -5, and -7 receptor activation, SMAD2 is phosphorylated and forms either homotrimers or heterotrimers with SMAD3 and SMAD4. These heterotrimers enter the nucleus and initiate gene transcription. Human SMAD2 is 467 amino acids (aa) in length. It contains one regulatory MH domain (aa 120-176) and a transactivation MH domain (aa 274-467). There are at least five utilized phosphorylation sites and one acetylation site at Lys19 that promotes transcriptional activity. There are four potential isoform variants. One shows a deletion of aa 79-108, while another contains the same deletion coupled to another deletion of aa 219-243. A third shows a deletion of aa 344-358, and a fourth is missing aa 221-225. Over aa 20-108, human SMAD2 shares 98% aa identity with mouse SMAD2.
运输条件	Blue Ice
存放说明	4℃
参考文献	Airway Inflammation and Remodeling Embryonic and Induced Pluripotent Stem Cell Markers EMT Induction Epithelial Stem Cell Transcription Factors Hematopoietic Stem Cell Transcription Factors and Regulators ICC / IHC Images: Smad2 Intracellular Signaling Molecules in Angiogenesis Negative Regulators of EMT Pluripotent Stem Cell Transcription Factors Smad Family Smad Transcription Factors TGF-beta Family TGF-beta Superfamily Modulators

参考图片

Smad2 in Human Pancreatic Cancer Tissue. Smad2 was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Goat Anti-Human Smad2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4037) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.