| 货号 | AF4036-SP |
| 别名 | EC 3.4.21; PROS; PROS1; protein S (alpha); protein Sa; PS21; PS22; PS23; PS24; PS25; PSA; vitamin K-dependent plasma protein S; vitamin K-dependent protein S |
| 反应种属 | Human |
| 应用 | Western Blot(0.2 µg/mL) Simple Western(2 µg/mL) |
| 目标/特异性 | Detects human PROS1 in direct ELISAs and Western blots. In these formats, approximately 20% cross‑reactivity with recombinant mouse Protein S is observed. |
| 使用方法 | Western Blot: 0.2 µg/mL Simple Western: 2 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5627 (Human); 19128 (Mouse); 81750 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Protein S controls hypoxic/ischemic blood-brain barrier disruption through the TAM receptor Tyro3 and sphingosine 1-phosphate receptor. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Protein S Ala42-Trp670 Accession # P07225 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Protein S is a 69 kDa, secreted glycoprotein that belongs to a general group of vitamin K-dependent proteins. It is synthesized principally by hepatocytes, circulates both free (40%) and bound to C4bBP (60%), interacts with protein C to suppress coagulation, and binds TAM family RTKs during immune regulation. Mature human Protein S is 634 amino acids (aa) in length. It contains one Gla domain (aa 42 ‑ 87), four EGF-like repeats (aa 117 - 283) and two laminin G-like domains (aa 299 ‑ 666). Over aa 42 - 670, human Protein S shares 83%, 80%, and 79% aa sequence identity with porcine, mouse, and canine Protein S, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Protein S/PROS1 by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Protein S/PROS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4036) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for Protein S/PROS1 at approximately 80 & 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of Human Protein S/PROS1 by Simple WesternTM. Simple Western lane view shows human plasma, loaded at 0.2 mg/mL. A specific band was detected for Protein S/PROS1 at approximately 106 kDa (as indicated) using 2 µg/mL of Goat Anti-Human Protein S/PROS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4036) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
