| 货号 | AF4029-SP |
| 别名 | Antigen NY-CO-25; heat shock 105kD beta; heat shock 105kDa protein 1; heat shock 105kDa/110kDa protein 1; Heat shock 110 kDa protein; heat shock protein 105 kDa; HSP105a; HSP105B; HSP105heat shock 105kD alpha; HSP110; KIAA0201DKFZp686M05240; NY-CO-25 | 全称 | Heat Shock Protein H1 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat HSPH1. This antibody is predicted to react with HSPH1 alpha and beta isoforms based on sequence identity of the immunogen. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10808 (Human); 288444 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant rat HSPH1 Val595-Asp733 Accession # Q66HA8 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The heat shock proteins (HSPs) are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. Heat Shock Proteins are ubiquitously expressed in all organisms. They are induced in response to various types of environmental stresses like heat, cold, and oxygen deprivation. Heat shock protein H1 (HSPH1), also known as HSP105 and HSP110, exists as two isoforms, alpha and beta. HSPH1 alpha consists of 858 amino acids whereas HSPH1 beta has 814 amino acids. alpha and beta have calculated molecular weights of 96.4 kDa and 92 kDa, respectively, but an apparent molecular weight of 105‑110 kDa in SDS‑PAGE. HSPH1 acts as a chaperone to prevent thermal aggregation in mammalian cells. HSPH1 alpha and beta isoforms share 94% identity. Rat HSPH1 has 92% and 97% identity to human and mouse HSPH1, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
HSPH1 in Rat Brain. HSPH1 was detected in perfusion fixed frozen sections of rat brain (cerebellum) using 5 µg/mL Human/Mouse/Rat HSPH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4029) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. | |
Detection of Human/Mouse/Rat HSPH1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, DA3 mouse myeloma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.1 µg/mL of Human/Mouse/Rat HSPH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4029) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for HSPH1 at approximately 105-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. |
