| 货号 | AF4027-SP |
| 别名 | bHLHc2; bHLHc2Class C basic helix-loop-helix protein 2; myf-5; myogenic factor 5 | 全称 | Myogenic Regulatory Factor 5 |
| 反应种属 | Human/Mouse |
| 应用 | Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects recombinant human MYF-5 in direct ELISAs and Western blots. |
| 使用方法 | Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4617 (Human); 17877 (Mouse); 299766 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human MYF-5 Met1-Leu255 Accession # P13349 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | MYF-5 (Myogenic factor 5; also bHLHc2) is a 37-39 kDa transcriptional activator that belongs to the bHLH superfamily of myogenic regulatory factors. It is found in embryonic skeletal muscle progenitors and neurons, and is induced by SHH and Wnt1. Expression of MYF-5 protein prompts nonmuscle cells to adopt a muscle precursor phenotype. Notably, neurons appear to block MYF-5 accumulation, thus preserving their unique phenotype. MYF-5 forms heterodimers with multiple molecules. When complexed to I-mfa, MYF-5 is retained in the cytoplasm and is inactive; when complexed to E12, MYF-5 is transcriptionally active. Phosphorylation regulates MYF-5 stability. Human MYF-5 is 255 amino acids (aa) in length. It contains one myogenic basic region (aa 1-83) followed by an HLH motif (aa 96-135). Full-length human MYF-5 (aa 1-255) shares 89% aa sequence identity with mouse MYF-5. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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MYF‑5 in C2C12 Mouse Cell Line. MYF‑5 was detected in immersion fixed C2C12 mouse myoblast cell line undifferentiated (upper panel) or differentiated with horse serum for 7 days (lower panel) using Goat Anti-Human/Mouse MYF‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4027) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
