| 货号 | AF4026-SP |
| 别名 | BHLHE8; circadian locomoter output cycles protein kaput; Class E basic helix-loop-helix protein 8; clock (mouse) homolog; clock homolog (mouse); EC 2.3.1.48; hCLOCK; KAT13D; KIAA0334bHLHe8circadian locomoter output cycles kaput protein | 全称 | Circadian Locomoter Output Cycles Kaput |
| 反应种属 | Human |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects human CLOCK in Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9575 (Human); 12753 (Mouse); 60447 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human CLOCK Glu108-Glu234 Accession # O15516 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | CLOCK is an essential regulator of circadian rhythms. CLOCK is a basic helix-loop-helix-PAS transcription factor that heterodimerizes with BMAL1. Together these transcription factors bind to E box elements (CACGTG) within the promoter of circadian genes (period1 and 2) stimulating their transcription. BMAL dimerization is necessary for CLOCK’s nuclear translocation, E box dependent transcription, phosphorylation, and degradation. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human CLOCK by Western Blot. Western blot shows lysates of PC-3 human prostate cancer cell line and HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 0.5 µg/mL of Human CLOCK Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4026) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CLOCK at approximately 95 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1. |
