| 货号 | AF4024-SP |
| 别名 | brix domain containing 2; Brix domain-containing protein 2; BRIX1; BRIXBXDC2; BRX1, biogenesis of ribosomes, homolog (S. cerevisiae); BXDC2; FLJ11100; ribosome biogenesis protein BRX1 homolog | 全称 | Brix Domain Containing 2 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human BRIX in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 55299 (Human); 67832 (Mouse); 294799 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human BRIX Ser131-Lys353 Accession # Q8TDN6 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | BRIX (Biogenesis of ribosomes in Xenopus; also BRIX domain-containing protein 2) is a 41 kDa (predicted) member of the BXDC2 family of proteins. It is found in nucleoli, and appears to participate in the formation of a complex associated with pre-rRNA and enzymes that generate 60S ribosomal subunits. Human BRIX is 353 amino acids (aa) in length. It is characterized by the presence of a BRIX domain that lies between aa 60‑249. There is one splice variant that shows a 17 aa substitution for aa 11‑27. Over aa 131‑353, human BRIX shares 93% aa identity with mouse BRIX. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
Detection of Human BRIX by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human BRIX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4024) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for BRIX at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
