| 货号 | AF4020-SP |
| 别名 | Zfp206; ZSCAN10 zinc finger and SCAN domain containing 10; ZSCAN10 | 全称 | Zinc Finger Protein 206/Zinc Finger and SCAN Domain Containing 10 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human ZNF206 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human ZNF24 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 84891 (Human); 332221 (Mouse); 302962 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human ZNF206 Met1-Val283 Accession # Q96SZ4 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | ZNF206 (zinc finger protein 206; also ZFP206) is an 80 kDa SCAN-zinc finger transcription factor that is active in embryonic stem cells and appears to positively regulate transcriptional regulators that maintain stem cell pluripotency. Human ZNF206 is 725 amino acids in length. It contains one SCAN box (aa 1‑71) that mediates oligomerization, followed by a proline-rich region (aa 104‑175) and 14 consecutive C2H2-type zinc fingers (aa 292‑719). Mouse ZNF206 apparently has multiple isoforms, none of which are well characterized. The human gene may also show alternate splicing. Human ZNF206 is 72% aa identical to mouse ZNF206. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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ZNF206 in BG01V Human Embryonic Stem Cells. ZNF206 was detected in immersion fixed BG01V human embryonic stem cells using Goat Anti-Human ZNF206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4020) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with tubulin (green). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips. |
