| 货号 | AF4014-SP |
| 别名 | BNSP; Bone sialoprotein 2; Bone sialoprotein; BSP 2; BSP II; BSP; BSP2; BSPII; BSP-II; Cell binding sialoprotein; IBSP; Integrin binding sialoprotein; SP II; SPII; SP-II | 全称 | Integrin-binding Sialoprotein |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects human IBSP/Sialoprotein II in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3381 (Human); 15891 (Mouse); 24477 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human IBSP/Sialoprotein II Phe17-Gln317 Accession # AAC95490 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | IBSP (integrin-binding sialoprotein; also BSP or bone sialoprotein (II)) is a 55‑75 kDa, secreted, variably glycosylated, monomeric noncollagenous member of the SIBLING family of extracellular matrix (ECM) proteins (1‑3). It is principally associated with the early stages of bone mineralization. BSP is synthesized as a 317 amino acid (aa) precursor that contains a 16 aa signal sequence and a 301 aa mature region (4‑6). The mature segment is divided into a basic N-terminus (aa 17‑62), a central region (aa 63‑233), and an acidic C‑terminus (aa 234‑317) (7). Functional segments associated with the mature molecule include a type I collagen binding domain (aa 19‑46), two non‑RGD cell binding sites (aa 30‑57 and 261‑281), an RGD alpha vbeta3 integrin‑binding site (aa 286‑288) and two potential hydroxyapatite (HAp) nucleation domains (aa 76‑83 and 151‑158) (3, 4, 8‑11). HAp formation requires a BSP nucleation site composed of at least eight consecutive glutamic acid residues and, likely, a contribution from a BSP‑associated co‑nucleator (10, 12). BSP is highly glycosylated, sulfated, and phosphorylated. Phosphorylation may impact HAp growth, while carbohydrate may regulate cell adhesion (1, 3, 13). Mature human BSP is 70%, 72%, 78%, and 72% aa identical to porcine, rat, canine, and mouse BSP, respectively. BSP is synthesized by megakaryocytes/platelets, osteoblasts, osteocytes, odontoblasts, osteoclasts, and bone marrow stromal cells (14‑17). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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