| 货号 | AF4008-SP |
| 别名 | EC 3.3.2.6; Leukotriene A(4) hydrolase; leukotriene A4 hydrolase; leukotriene A-4 hydrolase; LTA-4 hydrolase; LTA4; LTA4H |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human and mouse Leukotriene A4 Hydrolase/LTA4H in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4048 (Human); 16993 (Mouse); 299732 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Leukotriene A4 Hydrolase/LTA4H Pro2-Asp611 Accession # P09960 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Leukotrienes are a family of lipid mediators important in a variety of allergic and inflammatory reactions. Synthesized by leukocytes, these molecules are divided into two classes, the spasmogenic cysteinyl leukotrienes and LTB4, a classical chemoattractant (1). Encoded by the LTA4H gene, Leukotriene A4 Hydrolase catalyzes the conversion of unstable epoxide LTA4 to LTB4, which is the final and committed step in LTB4 biosynthesis. As a bifunctional zinc metalloenzyme, LTA4H also acts as an arginyl aminopeptidase (2). LTA4H is a drug target for anti-inflammation, and for cancer prevention and therapy (1, 3). The mature chain of human LTA4H consists of 610 amino acids (residues 2‑611). It is highly specific for LTA4, which also covalently modifies and inhibits the enzyme. The aminopeptidase activity is enhanced by monovalent anions (1). R&D Systems rhLTA4H corresponds to the mature chain, and is characterized by its arginyl aminopeptidase activity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human and Mouse Leukotriene A4 Hydrolase/LTA4H by Western Blot. Western blot shows lysates of Raji human Burkitts lymphoma cell line, CTLL‑2 mouse cytotoxic T cell line, and human tonsil tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse Leukotriene A4 Hydrolase/LTA4H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4008) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Leukotriene A4 Hydrolase/LTA4H at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
