Human PERK Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human PERK Affinity Purified Polyclonal Ab (25 UG)

货号： AF3999-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF3999-SP
别名	DKFZp781H1925; EC 2.7.11.1; EIF2AK3; eukaryotic translation initiation factor 2 alpha kinase 3; eukaryotic translation initiation factor 2-alpha kinase 3; hsPEK; PEK; PEKPERKPancreatic eIF2-alpha kinase; PRKR-like endoplasmic reticulum kinase; WRS
全称	Eukaryotic Translation Initiation Factor 4B
反应种属	Human
应用	Western Blot(1 µg/mL)
目标/特异性	Detects human PERK in Western blots.
使用方法	Western Blot: 1 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	9451 (Human); 13666 (Mouse); 29702 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Identification and characterization of PERK activators by phenotypic screening and their effects on NRF2 activation. Authors: Xie W, Pariollaud M, Wixted W, Chitnis N, Fornwald J, Truong M, Pao C, Liu Y, Ames R, Callahan J, Solari R, Sanchez Y, Diehl A, Li H PLoS ONE, 2015;10(3):e0119738. Species: Mouse Sample Type: Cell Lysates Application: WB Characterization of a novel PERK kinase inhibitor with antitumor and antiangiogenic activity. Authors: Atkins C, Liu Q, Minthorn E, Zhang S, Figueroa D, Moss K, Stanley T, Sanders B, Goetz A, Gaul N, Choudhry A, Alsaid H, Jucker B, Axten J, Kumar R Cancer Res, 2013;73(6):1993-2002. Species: Human Sample Type: Cell Lysates Application: WB Polycystin-2 down-regulates cell proliferation via promoting PERK-dependent phosphorylation of eIF2alpha. Authors: Liang G, Yang J, Wang Z Hum. Mol. Genet., 2008;17(20):3254-62. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human PERK Ala29-Gln230 Accession # Q9NZJ5
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	PERK, a type 1 ER membrane kinase, mediates eIF2 alpha phosphorylation at Ser51 during the UPR (unfolded protein response). Protein synthesis is inhibited, thereby reducing the burden of protein substrate for the ER folding and degradation mechanism. Phosphorylation of eIF2 alpha also selectively promotes the expression of UPR target genes such as Chop and BiP. PERK may also play a role in tumor cell adaptation to hypoxic stress by regulating the translation of angiogenic factors necessary for the development of functional microvessels. Mutations in PERK are responsible for the rare autosomal-recessive disorder, WRS (Wolcott-Rallison syndrome).
运输条件	Blue Ice
存放说明	4℃
参考文献	Translation Regulation by the Akt Pathway

参考图片

Detection of Human PERK by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human PERK Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3999) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PERK at approximately 130 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.