Human MCPH1 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human MCPH1 Affinity Purified Polyclonal Ab (25 UG)

货号： AF3998-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF3998-SP
别名	BRCT-repeat inhibitor of TERT expression 1; BRIT1; FLJ12847; MCT; microcephalin 1; Microcephalin; microcephaly, primary autosomal recessive 1
全称	Microcephalin 1
反应种属	Human
应用	Western Blot(1 µg/mL)
目标/特异性	Detects human, mouse and rat MCPH1 in Western blots.
使用方法	Western Blot: 1 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	79648 (Human); 244329 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Targeted Next-Generation Sequencing Identifies a Recurrent Mutation in MCPH1 Associating with Hereditary Breast Cancer Susceptibility. Authors: Mantere T, Winqvist R, Kauppila S, Grip M, Jukkola-Vuorinen A, Tervasmaki A, Rapakko K, Pylkas K PLoS Genet, 2016;12(1):e1005816. Species: Human Sample Type: Cell Lysates Application: WB A novel MCPH1 isoform complements the defective chromosome condensation of human MCPH1-deficient cells. Authors: Gavvovidis I, Rost I, Trimborn M, Kaiser F, Purps J, Wiek C, Hanenberg H, Neitzel H, Schindler D PLoS ONE, 2012;7(8):e40387. Species: Human Sample Type: Cell Lysates Application: WB Microcephalin and pericentrin regulate mitotic entry via centrosome-associated Chk1. Authors: Tibelius A, Marhold J, Zentgraf H, Heilig CE, Neitzel H, Ducommun B, Rauch A, Ho AD, Bartek J, Kramer A J. Cell Biol., 2011;185(7):1149-57. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human MCPH1 Met1-Lys250 Accession # Q8NEM0
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	MCPH1 (microcephalin 1), originally identified as an inhibitor of hTERT expression, has been implicated in DNA damage response. MCPH1 contains one N-terminal and two C-terminal BRCT domains. BRCT domains are found predominantly in cell cycle proteins responsive to DNA damage. MCPH1 forms irradiation-induced nuclear foci (IRIF) that colocalize with NBS1, 53BP1, MDC1, and ATM and are abolished with MCPH1 specific siRNA. MCPH1 also regulates the ATR pathway. It colocalizes with ATR and RPA and is required for the phosphorylation of RPA and Rad17. Defects in MCPH1 are the cause of primary microcephaly 1, an autosomal recessive neurodevelopmental disorder.
运输条件	Blue Ice
存放说明	4℃
参考文献	Checkpoint

参考图片

Detection of Human MCPH1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, CHP-100 human neuroblastoma cell line, PC-12 rat adrenal pheochromocytoma cell line, Rat-2 rat embryonic fibroblast cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human MCPH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3998) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MCPH1 at approximately 105 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.