| 货号 | AF3997-SP |
| 别名 | EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha; EIF2S1 eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa; EIF2S1 | 全称 | Eukaryotic Translation Initiation Factor 2 Alpha |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Simple Western(10 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat eIF2 alpha in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Simple Western: 10 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1965 (Human); 13665 (Mouse); 54318 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human eIF2 alpha Met1-Asp315 Accession # Q53XC0 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | elF2 alpha (eukaryotic translation initiation factor 2 alpha subunit) is a subunit of the elF2 protein, an important regulator of translation initiation. Phosphorylation of elF2 alpha on Ser 52 increases the affinity of elF2 alpha for elF2B, a guanine nucleotide exchange factor needed for the recycling of elF2-GDP to elF2-GTP. Reduction of elF2-GTP levels leads to the suppression of the overall rate of protein synthesis. Heme-regulated inhibitor (HRI), ER-resident protein kinase PERK, dsRNA activated protein kinase PKR, and the homologue of the S. cerevisiae protein kinase GCN2 are all elF2 alpha kinases. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human eIF2 alpha by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, MCF‑7 human breast cancer cell line, CTLL‑2 mouse cytotoxic T cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human eIF2 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3997) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for eIF2 alpha at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of Human eIF2 alpha by Simple WesternTM. Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for eIF2 alpha at approximately 42 kDa (as indicated) using 10 µg/mL of Goat Anti-Human eIF2 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3997) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody. |
