| 货号 | AF3995-SP |
| 别名 | DJ-1; DJ1FLJ34360; EC 3.4; FLJ27376; FLJ92274; Oncogene DJ1; Parkinson disease (autosomal recessive, early onset) 7; Parkinson disease protein 7; protein DJ-1 | 全称 | Parkinson Disease 7 |
| 反应种属 | Human |
| 应用 | Western Blot(0.2 µg/mL) Simple Western(2 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Park7/DJ-1 in Western blots. |
| 使用方法 | Western Blot: 0.2 µg/mL Simple Western: 2 µg/mL Immunohistochemistry: 5-15 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 11315 (Human); 57320 (Mouse); 117287 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Park7/DJ-1 Met1-Asp189 Accession # Q99497 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Park7, also known as DJ-1, is a cytoplasmic protein that belongs to the ThiJ/Pfp1/DJ-1 superfamily of highly conserved proteins that function as protein chaperones, catalases, proteases and kinases. Park7 is widely expressed in the brain as well as in peripheral tissues. It exists as a homodimer that can be localized in the cytoplasm, nucleus and mitochondria. Park7 is a redox-sensitive protein that has been ascribed various functions including that as a redox sensor and antioxidant protein. Mutations in Park7 are associated with a small percentage of hereditary early onset Parkinson’s disease. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Park7/DJ‑1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and A172 human glioblastoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Park7/DJ‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3995) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Park7/DJ‑1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Park7/DJ‑1 in HeLa Human Cell Line. Park7/DJ‑1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Park7/DJ‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3995) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
Detection of Human Park7/DJ‑1 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and A172 human glioblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Park7/DJ‑1 at approximately 28 kDa (as indicated) using 2 µg/mL of Goat Anti-Human Park7/DJ‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3995) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
