| 货号 | AF3975-SP |
| 别名 | FKHL16; FKHL16trident; forkhead box M1; forkhead box protein M1; Forkhead, drosophila, homolog-like 16; forkhead-like 16; Forkhead-related protein FKHL16; Hepatocyte nuclear factor 3 forkhead homolog 11; HFH-11; HFH11FOXM1B; HFH-11M-phase phosphoprotein 2; HNF-3; HNF-3/fork-head homolog 11; INS-1; MPHOSPH2; MPP-2; MPP2MPM-2 reactive phosphoprotein 2; PIG29; TGT3; Transcription factor Trident; Trident; WIN; Winged-helix factor from INS-1 cells | 全称 | Forkhead Box M1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human FoxM1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) FoxB2 and rhFoxJ1 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2305 (Human); 14235 (Mouse); 58921 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human FoxM1 Thr642-Gln763 Accession # Q08050 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | FoxM1 (Forkhead box protein M1; also FKHL16, HFH11 and WIN) is a 90-100 kDa member of the winged helix transcription factor gene family. It is expressed in proliferating and tumor cells and promotes the transition from G1 to S phase and G2 to M phase within the cell cycle. Human FoxM1 is 763 amino acids (aa) in length. It contains three PEST sequences (aa 22-34, 460-472 and 478-515), a forkhead DNA binding domain (aa 235-327), a Pro/Ser/Thr-rich region (aa 480-513) and a phosphorylation site at Ser704. There are at least two splice variants. The first shows a 38 aa insertion after Val423. This blocks FoxM1 transcriptional activation activity. The second variant shows a deletion of aa 326-340. Over aa 642-763, human FoxM1 shares 73% aa identity with mouse FoxM1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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FoxM1 in U2OS Human Cell Line. FoxM1 was detected in immersion fixed U2OS human osteosarcoma cell line using 10 µg/mL Sheep Anti-Human FoxM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3975) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
Detection of Human FoxM1 by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human FoxM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3975) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FoxM1 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
