| 货号 | AF3960-SP |
| 别名 | B3Galt6; B3GALT7; b3Gal-T7; B3GNT; B3GNT1; B3GN-T1; B3GN-T2; B3GNT-2; Beta-1,3-galactosyltransferase 7; Beta-1,3-GalTase 7; Beta-1,3-Gn-T1; Beta-1,3-Gn-T2; Beta-1,3-N-acetylglucosaminyltransferase 1; Beta-1,3-N-acetylglucosaminyltransferase 2; beta-1,3-N-acetylglucosaminyltransferase bGnT-1; beta-1,3-N-acetylglucosaminyltransferase bGnT-2; Beta3GalT7; Beta3Gal-T7; BETA3GNT; Beta3gnt1; Beta3Gn-T1; Beta3Gn-T2; Beta-3-Gx-T7; BGnT-1; BGNT2; BGnT-2; EC 2.4.1.-; UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 7; UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 7; UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1; UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 | 全称 | UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyl-transferase 1 |
| 反应种属 | Human/Rat |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human and rat beta -1,3-N-acetylglucosaminyltransferase 2/B3GNT2 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10678 (Human); 53625 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human beta -1,3-N-acetylglucosaminyltransferase 2/B3GNT2 Lys29-Cys397 Accession # Q9NY97 |
| 生物活性 | Human, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | B3-GNT (also B3GNT-2) is an 80 kDa (45 kDa without glycosylation) type II transmembrane Golgi enzyme that belongs to the glycosyltransferase 31 family of enzymes. It catalyzes the transfer of N-acetyl glucosamine from UDP-GlcNAc onto Gal beta 3(GlcNAc beta 6)GalNAc-mucin. It functions in the elongation and branching of O-linked oligosaccharide chains of mucin glycoproteins. Over aa 29-397, human B3GNT2 shares 88% aa sequence identity with rat B3GNT2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human and Rat beta -1,3-N-acetylglucosaminyltransferase 2/B3GNT2 by Western Blot. Western blot shows lysates of human brain tissue and rat testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human beta ‑1,3‑N‑acetylglucosaminyltransferase 2/B3GNT2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3960) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for beta ‑1,3‑N‑acetylglucosaminyltransferase 2/B3GNT2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
