| 货号 | AF394-SP |
| 别名 | Alternative macrophage activation-associated CC chemokine 1; AMAC-1; AMAC1CC chemokine ligand 18; AMAC-1Small-inducible cytokine A18; chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated); CKb7; DC-CK1; DC-CK1CC chemokine PARC; DCCK1Macrophage inflammatory protein 4; Dendritic cell chemokine 1; MIP4; MIP-4; MIP-4C-C motif chemokine 18; PARC; PARCPulmonary and activation-regulated chemokine; SCYA18chemokine (C-C), dendritic; small inducible cytokine A18; small inducible cytokine subfamily A (Cys-Cys), member 18, pulmonary andactivation-regulated |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Intracellular Staining by Flow Cytometry(2.5 µg/106cells) |
| 目标/特异性 | Detects human CCL18/PARC in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant human (rh) MIP-1 alpha and recombinant mouse (rm) MIP-1 alpha is observed and 10% cross-reactivity with rmMIP-1 beta, rhMIP‑1 beta, rhMPIF‑1, recombinant viral (rv) MIP‑I, rvMIP‑II, rhHCC-1, and rhMIP-1δ is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/106cells |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6362 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. High CCL18/PARC expression in articular cartilage and synovial tissue of patients with rheumatoid arthritis. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human CCL18/PARC Ala21-Ala89 Accession # P55774 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | CCL18, also known as alternative macrophage activation-associated CC chemokine (AMAC)-1, macrophage inflammatory protein (MIP)-4, and dendritic cell chemokine (DC-CK1), is a novel CC chemokine that is highly homologous to MIP-1 alpha (61% amino acid (aa) sequence identity). CCL18 cDNA encodes an 89 aa residue precursor protein with a 20 aa putative signal peptide that is cleaved to generate a 69 aa residue mature protein which lacks potential glycosylation sites. In vitro, CCL18 mRNA expression is induced in alternatively activated macrophages by Th2 cytokines such as IL-4, IL-10, and IL-13, and inhibited by IFN‑ gamma. CCL18 mRNA is also expressed by GM-CSF/IL-4-induced monocyte-derived dendritic cells. In vivo,CCL18 is highly expressed in lung and placenta but is not expressed in epidermal Langerhans cells. Recombinant CCL18 has been shown to chemoattract naive T cells but not monocytes or neutrophils. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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