| 货号 | AF3947-SP |
| 别名 | BDCA3; BDCA-3; CD141 antigen; CD141; Fetomodulin; THBD; THRM; thrombomodulin; TM |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/106cells) Immunohistochemistry(5-15 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects human Thrombomodulin in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse Thrombomodulin is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/106cells Immunohistochemistry: 5-15 µg/mL Immunoprecipitation: 25 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7056 (Human); 21824 (Mouse); 83580 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Thrombomodulin/BDCA-3 Ala19-Ser515 Accession # CAA29045 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Encoded by the THBD gene, Thrombomodulin is also known as CD141 antigen. The deduced amino acid (aa) sequence of human THBD (Accession # P07204) predicts a signal peptide (aa 1-18) and a mature chain (aa 19-575) that consists of following domains: C-type lectin (aa 31-169), EGF-like (aa 241-281, aa 284-324, aa 325-363, aa 365-405, aa 404-440, and aa 441-481), transmembrane (aa 516-539) and cytoplasmic (aa 540-575). The R&D Systems rhTHBD consists of aa 19-515, corresponding to the extracellular portion of the type I membrane protein. Predominantly synthesized by vascular endothelial cells, THBD inhibits coagulation and fibrinolysis (1-3). It functions as a cell surface receptor and an essential cofactor for active thrombin, which in turn activates protein C and thrombin-activatable fibrinolysis inhibitor (TAFI), also known as carboxypeptidase B2 (CPB2). Activated protein C (APC), facilitated by protein S, degrades coagulation factors Va and VIIIa, which are required for thrombin activation. Activated CPB2 cleaves basic C-terminal aa residues of its substrates, including fibrin, preventing the conversion of plasminogen to plasmin. In addition, THBD gene polymorphisims are associated with human disease and THBD plays a role in thrombosis, stroke, arteriosclerosis, and cancer (4). For example, increased serum levels of THBD, due to protease cleavage, have been associated with smoking, cardiac surgery, atherosclerosis, liver cirrhosis, diabetes mellitus, cerebral and myocardial infarction, and multiple sclerosis (5). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Thrombomodulin/BDCA‑3 in THP‑1 Human Cell Line by Flow Cytometry. THP‑1 human acute monocytic leukemia cell line was stained with Sheep Anti-Human Thrombomodulin/BDCA‑3 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3947, filled histogram) or control antibody (Catalog # 5‑001‑A, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). | |
Thrombomodulin/BDCA‑3 in Human Placenta. Thrombomodulin/BDCA‑3 was detected in immersion fixed paraffin-embedded sections of human placenta using Sheep Anti-Human Thrombomodulin/BDCA‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3947) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of syncytiotrophoblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
